The science behind Prolon

Rooted in longevity science

Prolon is the only nutrition program patented for longevity through cellular rejuvenation.

25+
Years of scientific research
Decades of studies on longevity and nutrition
41
Clinical trials
Ongoing or completed human trials
18
Leading research institutions
Including USC, Stanford, Mayo Clinic, and more
200+
Pending or issued patents
Backing the Prolon 5-Day FMD

Prolon's approach draws on the Fasting Mimicking Diet (FMD), a patented nutrition program built on research carried out at academic research centers and published in peer-reviewed scientific journals. Every study summarized on this page and in the research library below links to the original paper, and says plainly what it did and did not test.

Selected research

Six human studies from the research library, each with a plain-language summary and a link to the full paper.

Because of both the cellular reprogramming and metabolic reset benefits of the fasting mimicking nutri-technology, it is researched at Stanford, USC, Harvard, Mayo Clinic and 15 other top medical research centers, and published in 40+ top peer reviewed health journals such as Nature, Cell, Diabetologia and others.

Explore the research

Fasting-mimicking diet and markers/risk factors for aging, diabetes, cancer, and cardiovascular disease

Human randomized controlled trial

Sci Transl Med, 2017

In a 100-person randomized trial, the authors reported that three monthly FMD cycles reduced body weight and body fat compared with a usual diet.

Fasting mimicking diet cycles versus a Mediterranean diet and cardiometabolic risk in overweight and obese hypertensive subjects: a randomized clinical trial

Human randomized controlled trial

NPJ Metab Health Dis, 2023

In a randomized trial in adults with hypertension and excess weight, four monthly FMD cycles and four months of a Mediterranean diet produced comparable reductions in several cardiometabolic risk factors.

Fasting-mimicking diet causes hepatic and blood markers changes indicating reduced biological age and disease risk

Human study, secondary analysis

Nat Commun, 2024

In blood-sample analysis from two human studies, three consecutive monthly cycles of the Prolon 5-Day FMD were associated with a median reduction of approximately 2.5 years in biological age.

Fasting-Mimicking-Diet does not reduce skeletal muscle function in healthy young adults: a randomized control trial

Human randomized controlled trial

Eur J Appl Physiol, 2022

A randomized trial in 24 young men found FMD cycles did not reduce muscle force or muscle volume.

The Effects of a Fasting Mimicking Diet on Skin Hydration, Skin Texture, and Skin Assessment: A Randomized Controlled Trial

Human randomized controlled trial

J Clin Med, 2023

In a 45-woman randomized trial, skin hydration rose after three monthly FMD cycles, and skin texture held steady while roughness increased in the control group.

An Intermittent Fasting Mimicking Nutrition Bar Extends Physiologic Ketosis in Time Restricted Eating: A Randomized, Controlled, Parallel-Arm Study

Human randomized controlled trial

Nutrients, 2021

A randomized trial found an FMD energy bar did not interrupt ketosis compared with continuing a water-only fast.

How the research is built

Prolon's research draws on several connected lines of scientific inquiry. Each one asks a different kind of question, and together they shape how new studies are designed and interpreted.

Juventology research

The study of the biological mechanisms of aging and rejuvenation, including how specific cells and tissues respond to interventions such as periodic fasting.

Epidemiological research

Population-level research that looks for patterns between diet, lifestyle and health outcomes across large groups, generating the questions that controlled trials then test directly.

Clinical studies

Randomized, controlled trials in humans that test a specific intervention, such as a fasting-mimicking diet cycle, against a comparison group to evaluate effectiveness and safety.

Centenarian studies

Research into the diets, habits and biology of populations with an unusually high number of people living past 100, used to generate hypotheses about healthy aging.

Complex systems analysis

Methods that model how diet, cellular damage and aging interact across multiple systems in the body, rather than looking at any one marker or pathway alone.